Fig. 6

Substance P (SP) and calcitonin gene-related peptide (CGRP) immunostaining of the dorsal root ganglion (DRG) in the control (a, b), vehicle-treated (vehicle c, d), and nerve growth factor gene-treated (NGF e, f) groups at 4 weeks after 1 week of pyridoxine injection. In the control group, SP immunoreactive neurons are mainly detected in the small- and medium-sized neurons, while CGRP immunoreactive neurons in the small-, medium-, and large-sized neurons are well detected in the DRG. Number of SP immunoreactive neurons is similarly detected in all groups, while the number of CGRP immunoreactive neurons is decreased in the small- and medium-sized neurons of vehicle-treated group and this reduction is significantly ameliorated in the NGF group. Scale bar = 100 μm. g and h: Relative number as a % of small-, medium-, and large-sized neurons of the control group in the DRG (n = 4 per group; *P < 0.05, significantly different from the control group; ^# P < 0.05, significantly different from the vehicle group). The bars indicate the mean ± SEM