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Fig. 3 | BMC Neuroscience

Fig. 3

From: Limb remote ischemic postconditioning protects cerebral ischemia from injury associated with expression of HIF-1α in rats

Fig. 3

Immunohistochemical staining for HIF-1α in ischemic cerebral cortex at 3d reperfusion after MCAO injury. a Immunofluorescence double staining for HIF-1α/NeuN in neurons treated by LRIP. b Immunofluorescence double staining for HIF-1α/GFAP in astrocytes treated by LRIP. The immunofluorescence showed nucleus were stained by DAPI with blue color and HIF-1α were stained with green color, neurons were stained by NeuN and astrocytes were stained by GFAP with red color, and the co-expression of NeuN with HIF-1α within the same cell showed blue color (merged), the co-expression of GFAP with HFI-1α within the same cell showed white color (merged). The arrow showed double staining for HIF-1α/NeuN or HIF-1α/GFAP (scale bar = 20 μm). c HIF-1α immunoreactivity was marked with brown color in cytoplasm (scale bar = 20 μm)

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