Fig. 4

Construction of Synaptotagmin1 shRNA. a Fluorescent images of Venus-Synaptotagmin1 in HEK293T cells (left) and Venus-Synaptotagmin1 and Synaptotagmin1 shRNA in HEK293T cells (right). Scale bars = 10 μm. b The graph shows the relative fluorescence of Synaptitagmin1 cells after transfection of shRNA constructs. *p < 0.005 by Student’s t test against the control. c, d Western blot analysis of transfected HEK293T. Cells were transfected with Venus-Synaptotagmin1