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Table 1 Primer sets for amplification of the open reading frame of zebrafish wdr81
| Primer sequence | Annealing temperature (°C) | Expected amplicon size (bp) | |
|---|---|---|---|
| Pair 1 | |||
| F | 5′-GCAAACAGTGCAGAGCTTCTT-3′ | 60 | 897 |
| R | 5′-GCTGCTCATCAACTGCAATATC-3′ | ||
| Pair 2 | |||
| F | 5′-CCTATCCACCTGCTCAGCTC-3′ | 60 | 874 |
| R | 5′-AACATGGCTGCATAGCACAG-3′ | ||
| Pair 3 | |||
| F | 5′-CCAGATCTTGGTGGACCAGT-3′ | 60 | 909 |
| R | 5′-TCTGTGAAGCATGGCAGTTC-3′ | ||
| Pair 4 | |||
| F | 5′-TTGGTTTGGTTGTGTCTCCA-3′ | 60 | 875 |
| R | 5′-ATACCAGGCCGCATAAACAG-3′ | ||
| Pair 5 | |||
| F | 5′-CTCCATCATGCACTGGACAC-3′ | 60 | 920 |
| R | 5′-CCAACTGTTGTTGCTCCAGA-3′ | ||
| Pair 6 | |||
| F | 5′-GCCACATCTTCTGGCAAAGT-3′ | 55 | 979 |
| R | 5′-TGAGTACCACAGCACCCAAA -3′ | ||
| Pair 7 | |||
| F | 5′-GAGACCAGACTGCAAGACCAG-3′ | 55 | 698 |
| R | 5′-ACTTGCTCGTTCGTGGTAAGA-3′ | ||
| Pair 8 | |||
| F | 5′-GCAGAGTGCACATACCTGGA-3′ | 60 | 704 |
| R | 5′-TGGAAGTGGAAGTGGGAGTC-3′ | ||
| Pair 9 | |||
| F | 5′-AACAGGACCTTCCACGTAGC-3′ | 60 | 896 |
| R | 5′-TGTGCTGTCCAGAATGGAGT-3′ | ||
| Pair 10 | |||
| F | 5′-CACAAGCCACTCCACCAGTA-3′ | 60 | 429 |
| R | 5′-CGCGGAGGTTGTAAGTTCTC-3′ | ||
