Skip to main content

Table 1 Primer sets for amplification of the open reading frame of zebrafish wdr81

From: Characterization of a novel zebrafish (Danio rerio) gene, wdr81, associated with cerebellar ataxia, mental retardation and dysequilibrium syndrome (CAMRQ)

  Primer sequence Annealing temperature (°C) Expected amplicon size (bp)
Pair 1
 F 5′-GCAAACAGTGCAGAGCTTCTT-3′ 60 897
 R 5′-GCTGCTCATCAACTGCAATATC-3′
Pair 2
 F 5′-CCTATCCACCTGCTCAGCTC-3′ 60 874
 R 5′-AACATGGCTGCATAGCACAG-3′
Pair 3
 F 5′-CCAGATCTTGGTGGACCAGT-3′ 60 909
 R 5′-TCTGTGAAGCATGGCAGTTC-3′
Pair 4
 F 5′-TTGGTTTGGTTGTGTCTCCA-3′ 60 875
 R 5′-ATACCAGGCCGCATAAACAG-3′
Pair 5
 F 5′-CTCCATCATGCACTGGACAC-3′ 60 920
 R 5′-CCAACTGTTGTTGCTCCAGA-3′
Pair 6
 F 5′-GCCACATCTTCTGGCAAAGT-3′ 55 979
 R 5′-TGAGTACCACAGCACCCAAA -3′
Pair 7
 F 5′-GAGACCAGACTGCAAGACCAG-3′ 55 698
 R 5′-ACTTGCTCGTTCGTGGTAAGA-3′
Pair 8
 F 5′-GCAGAGTGCACATACCTGGA-3′ 60 704
 R 5′-TGGAAGTGGAAGTGGGAGTC-3′
Pair 9
 F 5′-AACAGGACCTTCCACGTAGC-3′ 60 896
 R 5′-TGTGCTGTCCAGAATGGAGT-3′
Pair 10
 F 5′-CACAAGCCACTCCACCAGTA-3′ 60 429
 R 5′-CGCGGAGGTTGTAAGTTCTC-3′