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Fig. 2 | BMC Neuroscience

Fig. 2

From: Characterization of a novel zebrafish (Danio rerio) gene, wdr81, associated with cerebellar ataxia, mental retardation and dysequilibrium syndrome (CAMRQ)

Fig. 2

Agarose gel electrophoretic separation of overlapping amplicons of the zebrafish wdr81 open reading frame. Amplification of the open reading frame from 24 hpf embryo cDNA and adult brain cDNA resulted in one amplicon per reaction indicating that there is one open reading frame of zebrafish wdr81. Since the experiment was designed based on the predicted sequence, this result also confirms the prediction. a Amplification of 24 hpf embryo cDNA. b Amplification of adult brain cDNA. Lanes 1, 3, 5, 7, 9, 11, 13, 15, 17 and 19 were loaded with the PCR products of primer pairs 1–10, respectively. Lanes 2, 4, 6, 8, 10, 12, 14, 16, 18 and 20 were loaded with the PCR products of −RT controls amplified with primer pairs 1–10, respectively. Lanes indicated as M are loaded with pUC mix DNA Marker (SM0301, Thermo Scientific)

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