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Fig. 7 | BMC Neuroscience

Fig. 7

From: Navigational choice between reversal and curve during acidic pH avoidance behavior in Caenorhabditis elegans

Fig. 7

Acidic pH avoidance behavior of rgs-3(vs19), egl-3(n159) and egl-3(n729) mutants. a Boxplot of distance of reversal and curve avoidance relative to the acidic color border in glutamatergic mutants. The acidic pH gradient was formed using 30 mM HCl on 5 mM potassium phosphate buffer (see Additional file 3: Fig. S4 for steepness). Wild-type, rgs-3(vs19), egl-3(n159), egl-3(n729) strains were examined. b Boxplot of distance of reversal and curve avoidance of the egl-3mutants. The acidic pH gradient was formed using 10 mM HCl on 5 mM potassium phosphate buffer. Wild-type, egl-3(n159), egl-3(n729) strains were examined. c Boxplot of distance of reversal and curve avoidance relative to the acidic color border in rgs-3(vs19) mutants. The acidic pH gradient was formed using 30 mM HCl on 5 mM potassium phosphate buffer. Wild-type, rgs-3(vs19), rgs-3(vs19); ASH::rgs-3(wt) [ASH(+)], and rgs-3(vs19); ASK::rgs-3(wt) [ASK(+)] strains were examined. ASH(+) and ASK(+) are transgenic strains harboring a transgene driving ASH- and ASK-specific expression of the wild-type rgs-3 gene, respectively. Statistical significances of difference were examined using Student’s t-test. Asterisk indicates a significant difference between wild-type and mutants (*p < 0.05, **p < 0.01). Dagger indicates a significant difference between the rgs-3(vs19) and ASH(+), ASK(+) strain (†† p < 0.01)

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