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Fig. 7 | BMC Neuroscience

Fig. 7

From: Novel transcripts reveal a complex structure of the human TRKA gene and imply the presence of multiple protein isoforms

Fig. 7

TRKAκ isoform’s localization into the nucleus. a immunocytochemical analysis revealing TRKAκ-V5-His and TRKAII-V5-His localization in primary rat neurons and HEK293 cells. DNA was stained with DAPI. To mark glycoproteins embedded in the plasma membrane, Alexa Fluor 488-conjugated concanavalin A (con A) was used, and TRKA-V5-His proteins were visualized with anti-V5-tag antibodies and secondary Alexa Fluor 568-conjugated antibodies. Scale bar 5 µm. b Nuclear accumulation of EGFP-fused TRKAκ in HEK293 cells. Cells were transfected with constructs encoding TRKAκ-EGFP or EGFP, and mCherry-NLS (the marker for nucleus). Live cells were imaged with a confocal microscope. Scale bar 5 µm. c HEK293 were fixed and subjected to immunocytochemistry 24 h p.t. of expression constructs encoding TRKAII-V5-His, TRKAκ-V5-His, TRKAκ-EGFP or EGFP. The cells were visualized with confocal microscopy and counted by determining the localization of given protein of interest in n number of cells. The graph shows pooled results of two independent experiments. C = N, the signal was observed uniformly in cytosol and nucleus; C > N, the signal was greater in cytosol than in nucleus; C < N, the signal was greater in nucleus than in cytosol

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