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Fig. 4 | BMC Neuroscience

Fig. 4

From: Novel transcripts reveal a complex structure of the human TRKA gene and imply the presence of multiple protein isoforms

Fig. 4

Detection of endogenous TRKA protein in human SH-SY5Y and rat PC12 cell line. a 50 µg of lysates of PC12 cells transfected (48 h) with siRNAs and SH-SY5Y cells was separated on SDS-PAGE. Western blot analysis was performed using Cell Signaling anti-TRK [epitope in the intracellular region (ICD) of TRK proteins; #4609] and Millipore anti-TRKA [epitope in the extracellular region (ECD); #06-574] antibodies. b PC12 cells were transfected (48 h) with siRNAs and treated for 5 min with NGF. Mock-treated cells were used as control. 60 µg of lysates was subjected to SDS-PAGE and western blotting with antibody against phosphorylated TRKA (Cell Signaling anti-pTRKA; #9141). GAPDH was used to validate the loading efficiency. siScr unspecific scrambled siRNA, siTRKA rat TRKA specific siRNA, neg mock treated cells

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