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Table 1 Oligonucleotides used for generation of PFN1 T109M, T109A and T109D by site directed mutagenesis

From: Profilin 1 with the amyotrophic lateral sclerosis associated mutation T109M displays unaltered actin binding and does not affect the actin cytoskeleton

Mutation Sequence
T109M 5′-CTGTCACCAAGACTGACAAGATGCTAGTCCTGCTGATGGGC-3′
5′-GCCCATCAGCAGGACTAGCATCTTGTCAGTCTTGGTGACAG-3′
T109A 5′-CTGTCACCAAGACTGACAAGGCGCTAGTCCTGCTGATGGGC-3′
5′-GCCCATCAGCAGGACTAGCGCCTTGTCAGTCTTGGTGACAG-3′
T109D 5′-CTGTCACCAAGACTGACAAGGATCTAGTCCTGCTGATGGGC-3′
5′-GCCCATCAGCAGGACTAGATCCTTGTCAGTCTTGGTGACAG-3′