Table 1 Oligonucleotides used for generation of PFN1 T109M, T109A and T109D by site directed mutagenesis

T109M

5′-CTGTCACCAAGACTGACAAGATGCTAGTCCTGCTGATGGGC-3′

5′-GCCCATCAGCAGGACTAGCATCTTGTCAGTCTTGGTGACAG-3′

T109A

5′-CTGTCACCAAGACTGACAAGGCGCTAGTCCTGCTGATGGGC-3′

5′-GCCCATCAGCAGGACTAGCGCCTTGTCAGTCTTGGTGACAG-3′

T109D

5′-CTGTCACCAAGACTGACAAGGATCTAGTCCTGCTGATGGGC-3′

5′-GCCCATCAGCAGGACTAGATCCTTGTCAGTCTTGGTGACAG-3′