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Fig. 4 | BMC Neuroscience

Fig. 4

From: Profilin 1 with the amyotrophic lateral sclerosis associated mutation T109M displays unaltered actin binding and does not affect the actin cytoskeleton

Fig. 4

The T109M mutation of PFN1 does not change the F/G-actin ratio in HEK293 cells. Upper panel F/G-actin ratios of HEK293 cells overexpressing wildtype and mutant V5-PFN1. F- and G-actin fractions of the respective cell lysates were separated by ultracentrifugation and actin levels analyzed by Western blotting and densitometry (n = 5; bars indicate mean ± SEM; * p ≤ 0.05 in a two tailed student’s t test). Lower panel Representative Western blot of F- and G-actin fractions used for densitometric analysis

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BMC Neuroscience

ISSN: 1471-2202

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