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Fig. 2 | BMC Neuroscience

Fig. 2

From: spargel, the PGC- homologue, in models of Parkinson disease in Drosophila melanogaster

Fig. 2

Tissue specific srl expression in the Drosophila melanogaster eye results in a reduction in both ommatidia and bristle number. a Scanning electron micrographs of D. melanogaster eyes taken at a horizontal field width of 500 µm. Genotypes are as follows: (I) GMR-Gal4/UAS-lacZ 25 °C, (II) GMR-Gal4/srl-RNAi 1 (UAS-srl HMS00857) 25 °C, (III) GMR-Gal4/srl-RNAi 2 (UAS-srl HMS00858) 25 °C, (IV) GMRGal4/srl-EY (UAS-srl EY05931) 25 °C, (V) GMR-Gal4/UAS-lacZ 29 °C, (VI) GMR-Gal4/srl-RNAi 1 (UAS-srl HMS00857) 29 °C, (VII) GMR-Gal4/srl-RNAi 2 (UAS-srl HMS00858) 29 °C, (VIII) GMRGal4/srl-EY (UAS-srl EY05931) 29 °C. Images were taken with a FEI MLA 650. b Flies show a decrease in the mean number of ommatidia present when srl-RNAi 1 (UAS-srl HMS00857) and srl-RNAi 2 (UAS-srl HMS00858) are driven with the GMR-Gal4 driver in both standard conditions (25 °C) and at a higher temperature (29 °C). Flies show a slight but not significant decrease in ommatidia number when srl-EY is expressed in a tissue specific manner (UAS-srl EY05931) in both standard conditions (25 °C) and at a higher temperature (29 °C). c Flies show a strong decrease in bristle number in standard conditions (25 °C) when srl-RNAi 1 (UAS-srl HMS00857), srl-RNAi 2 (UAS-srl HMS00858) and srl-EY (UAS-srl EY05931) are expressed in D. melanogaster eyes. At a higher temperature (29 °C) srl-EY causes an increase in number of bristles formed compared to lacZ controls, however, this is not statistically significant (UAS-lacZ). Comparisons were measured using a one-way ANOVA and significance was tested using a Tukey post hoc test, n = 10. *P < 0.05, **P < 0.01, ***P < 0.001

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