Fig. 7

Double staining for RIP3 and TUNEL on the injured side in transverse sections obtained 3 days after hemisection. The numbers of RIP3-expressing and TUNEL-positive cells were obviously increased in the injured spinal cord (a–c). At higher magnification (d–f), the TUNEL-positive cells exhibiting shrunken or fragmented nuclei (arrowheads), a hallmark of apoptotic cell death, rarely expressed RIP3. Scale bars 100 μm (a–c), 20 μm (d–f). The schematic drawing illustrates the location of the micrographs (g)