Fig. 2

Dcx is up-regulated upon Dicer deletion in hippocampal new born neurons. a, b Representative pictures of the immunostaining for Dcx and GFP on hippocampal regions of Dicer cKO mice transduced with Cre-recombinase. a Dicer flox/+ mice (control) 28 dpi. b Dicer flox/flox mice (experiment) 28 dpi. Transduced cells are GFP positive (green) and identified by white circles. Arrowheads indicate Dcx-expressing (red) among the Cre-transduced (green) cells. Single channel and overlay pictures are shown. Scale bar 25 µm. c Quantification of Dcx/GFP double positive cells among the virus-transduced cells. Bars show the average ± the standard error of the mean (n = 4). At least 100 virus-transduced cells per group of four animals were analyzed. Two-tailed t test was applied for statistical analysis. Asterisks indicate statistically relevant differences; p < 0.05