Fig. 7

Oxycodone increases ATF4 in purkinjie cells and PDGFRα expression in granular layer of cerebellum. a Immunohistochemical analysis of hsp70, BiP, phosphorylated eIF2α, and ATF4 in Purkinje cells. Upper panels representative images of Purkinje cells (arrow) of rats treated with water (W) or oxycodone (O). Scale bar denotes 20 μm. WM white matter; GL granule layer; ML molecular layer. Lower bars statistical analysis of the data. Open bars water samples; gray filled bars oxycodone samples. The intensity hsp70, BiP, phosphorylated eIF2α, and ATF4 staining in Purkinje cells was analyzed using the Q method. The graphs represent the mean value of intensities measured in brain slides obtained from three animals for each treatment (±SEM). In each slide, at least 3 fields containing 6–18 Purkinjie cells each were investigated. Statistical analysis was performed using Student’s t test. Hsp70, p < 0.01; BiP, p < 0.01; P-eIF2α, p < 0.001; ATF4, p < 0.001. b Upper panels immunohistochemical analysis of PDGFRα and oxidized DNA (8H) in granular layers (GL) of rats treated with water (W) or oxycodone (O). Scale bar denotes 20 μm. ML molecular layer. Lower bars statistical analysis of the data. Open bars water samples; gray filled bars oxycodone samples. The intensity of PDGFRα and oxidized DNA staining in granule cells was analyzed using Density method. The graphs represent the mean value of intensities measured in brain slides obtained from three animals for each treatment (±SEM). In each slide, at least 3 fields were investigated. Data was analyzed by Student’s t test. PDGFRα, p < 0.001; 8H, p < 0.01