Fig. 5

Oxycodone increases ATF4 and PDGFRα expression in corpus collosum and striatum. a Immunohistochemical analysis of BiP, ATF4, and PDGFRα in corpus collosum. Upper panels representative images of corpus collosum areas of rats treated with water (W) or oxycodone (O). Scale bar denotes 50 μm. Lower bars statistical analysis of the data. Open bars water samples; gray filled bars oxycodone samples. The graphs represent the mean value of percentage of cells expressing BiP, ATF4, or PDGFRα in corpus collosum areas obtained from three animals (±SEM). In each slide, at least 5 fields containing 25–70 cells were investigated. Statistical analysis was performed using Student’s t test. BiP, p < 0.001; ATF4, p < 0.001, PDGFRα, p < 0.001. b Immunohistochemical analysis of ATF4, PDGFRα, and oxidized DNA (8H) in striatum areas of rats treated with water (W) or oxycodone (O). Scale bar denotes 20 μm. Arrows point to the blood vessels