Skip to main content

Table 6 In vivo and in vitro exposure paradigms converge with human data on same cellular functions

From: Alterations in serum microRNA in humans with alcohol use disorders impact cell proliferation and cell death pathways and predict structural and functional changes in brain

Humans Rat drinking models Mouse neural stem cells
Serum 3 week daily serum 3 week binge serum FGF2 + EtOH-treated TGFβ1 + EtOH-treated
Functional group p-value # Mol Functional group p-value # Mol Functional group p-value # Mol Functional group p-value # Mol Functional group p-value # Mol
Cellular developmenta 1.77 × 10−3 3 Cellular developmenta 1.10 × 10−10 32 Cellular developmenta 8.32 × 10−18 35 Cellular developmenta 2.64 × 10−12 49 Cell death and survivala 4.37 × 10−7 12
Cell death and survivala 2.36 × 10−3 4 Cell growth and proliferationa 1.10 × 10−10 27 Cell growth and proliferationa 8.32 × 10−18 34 Cell growth and proliferationa 9.86 × 10−5 42 Cellular developmenta 1.33 × 10−5 13
Cell morphology 4.72 × 10−3 1 Cell death and survivala 5.65 × 10−8 26 Cell death and survivala 6.89 × 10−10 27 Cell cycle 1.82 × 10−4 18 Cell growth and proliferationa 1.33 × 10−5 13
Cell function and maintenance 4.71 × 10−3 1 Cellular movement 1.15 × 10−5 18 Cell cycle 1.68 × 10−7 16 Cellular movement 1.82 × 10−4 27 Cell cycle 1.88 × 10−5 9
Cellular growth and proliferationa 1.47 × 10−2 2 Cell cycle 1.14 × 10−4 12 Cellular assembly and organization 5.94 × 10−5 5 Cell death and survivala 1.07 × 10−3 17 Cell morphology 1.85 × 10−4 5
  1. Ingenuity IPA analysis of significantly changed miRNAs found using RNA-sequencing (Tiers A and B) show top functional groups affected by ethanol in each experiment. Different ethanol exposure paradigms, both in vitro (in neural stem cells) and in vivo (in humans and rats), show complete convergence for 3 of the top 5 functional groups (a) identified by IPA as affected by ethanol