Expression profiling of the ubiquitin conjugating enzyme UbcM2 in murine brain reveals modest age-dependent decreases in specific neurons

Table 1 UbcM2 expression is decreased in the neurons of specific substructures of the mouse brain

Brain structure	Relative UbcM2 expression	UbcM2 expression % change in aged mice	P value
CA1	0.776	−11.518	0.0590
CA2	0.711	−9.714	0.1500
CA3	0.801	−7.614	0.1800
Caudate putamen	0.553	−6.471	0.4500
Cerebellum-granule layer	0.951	−14.530	0.0032
Cingulate ctx	0.679	1.317	0.8900
Dentate gyrus	0.630	1.806	0.8100
Facial nu	0.955	1.957	0.6900
Hypothalamus-lateral	0.919	6.637	0.1800
Inferior colliculus	0.687	−7.692	0.2800
Motor ctx-primary	0.951	−11.111	0.0260
Motor ctx-secondary	0.984	−5.372	0.2600
Nucleus accumbens	0.634	−7.051	0.3500
Olfactory nu	0.927	−10.526	0.0410
Pontine nu	0.976	7.083	0.1400
Reticular formation-lateral	0.976	−0.917	0.8500
Sensory ctx-primary	0.825	−10.837	0.0560
Spinal trigeminal nu	1.000	−8.943	0.0600
Superior colliculus	0.699	−14.535	0.0320
Thalamus-laterodorsal	0.789	−3.247	0.5800
Thalamus-ventral	0.720	−0.847	0.9000
Visual ctx-secondary	0.923	−11.454	0.0290

The expression levels of UbcM2 in the brains of young and aged mice were measured and compared by average cellular α-UbcM2-derived fluorescence intensity in NeuN-positive nuclei. A 5.5 % global decrease of UbcM2 expression was detected in aged versus their strain-matched, younger counterparts. The italic numbers indicate decreases of UbcM2 expression of 10 % or greater in particular substructures of the brain

ISSN: 1471-2202