Fig. 2

UbcM2 is expressed in neurons and oligodendrocytes but is undetectable in most microglia and astrocytes. Representative images from stained 7 μm, paraffin-embedded sagittal C57BL/6 brain sections of dorsal cortex and CA1, along with cerebellum (A, B) or thalamus (C, D). A Nuclear neuronal marker NeuN (green) and UbcM2 (red) with yellow indicating colocalization in the merged images (c, f, i). B–D Co-staining with cell type-specific marker CNPase for oligodendrocytes (B), Iba1 for microglia (C), or GFAP for astrocytes (D) shown in green with Hoechst (white, a, c, e) to mark nuclei or with UbcM2 (red, b, d, f). White arrowheads indicate oligodendritic (B), microglial (C), or astrocytic (D) nuclei. 60×, size bar 10 µm