Fig. 1

Validation of α-UbcM2 antibody. A HeLa cells were treated with 12 pmol of control siCON siRNA (a, b) or UbcM2-specific siRNA (c, d) for 72 h before fixation and immunostaining with α-UbcM2 (b, d). Nuclei were counterstained with DAPI (a, c). 60×, size bar 10 µm. B RPE-1 cells were treated with siCON (left lane) or siUbcM2 (right lane) for 72 h and solubilized for western blot analysis with α-UbcM2. Anti-β-tubulin is used as a loading control. C UbcM2^Flox/Flox MEFs expressing an inducible Cre recombinase were fixed and labeled with DAPI (a), α-UbcM2 (b), and α-Cre (c). 3 of the 4 cells express Cre and lose UbcM2 expression whereas the non-Cre expressing cell retains UbcM2 expression. D Cell lysates prepared from mouse cerebellum (CB) and cortex (Ctx) were analyzed by α-UbcM2 western blot analysis