Galanin immunohistochemistry. Galanin immunostaining of dorsal hippocampal CA1/CA2 regions in one control (a–c) and one high fat-fed (d–f) rat after 72 h. Scale bars set to 30 μm. a, d show DAPI staining in blue, b, e show galanin staining in green, and in c, f the images are merged. Quantification of GFP signal for high fat fed (n = 3) and control fed (n = 4) rats using Image J software (g). Light bars indicate control diet and dark bars indicate high fat diet. Statistics were performed using t test.