Fig. 2
From: Receptor interacting protein 3-induced RGC-5 cell necroptosis following oxygen glucose deprivation
Ratio of necrotic cells is reduced following Nec-1 pre-treatment by OGD. a Normal control cells; b RGC-5 cell necrosis after OGD; c RGC-5 cells were pre-treated with Nec-1 (10 μM) to block necroptosis for 24 h before OGD and analysis of necroptotic cells. Cells were stained with Annexin fluorescein isothiocyanate and PI, and analyzed by FACS using FL1 (Annexin V) and FL3 (PI) channels. d The statistical analysis of RGC-5 necrosis, * vs CTL, P < 0.05; # vs Nec-1 pre-treatment, P < 0.05.