mRNA and microRNA analysis reveals modulation of biochemical pathways related to addiction in the ventral tegmental area of methamphetamine self-administering rats

Table 1 Differential expression of mRNA from methamphetamine self-administration rats

Gene name	Fold change (log2)	p-value	miRNA target^a
Upregulated
Endosomes
Slc9a6	−0.51	0.0025	miR-181a/d, 16, 195, 124
Mtpn	−1.00	0.0028	miR-124, 9, 181, 140, 143, 26a, let-7
Myo18b	−0.53	0.0049
Cell signaling
Ankh	−0.56	9.30E−05	miR-9
Ppm1h	−0.56	0.00087	miR-125a-5p/b-5p, 351
Gpr64	−0.60	0.0012	miR-23a/b
Cd47	−0.52	0.0016	miR-181d, 9
Neurite growth/extension
Ntn1	−0.75	0.0010	let-7d/e, miR-27a/b, 20a, 106b
LOC691277	−0.52	0.0040
Neuroprotection
Pex3	−0.64	0.00052	miR-30b-5p/c/d
Coa5/6330578E17Rik	−0.87	0.00024
Ret	−0.88	0.0023	miR-23a/b, 128, 27a/b, 125a-5p/b-5p
Hsp90ab1	−0.82	0.0030
RNA processing
Hnrnpa3	−0.62	0.00034	miR-221, 222, 206
Rpp30	−0.55	0.0022
Rpl19	−0.94	0.0037
LOC100359671	−0.55	0.0049
Membrane transporters/receptors
Olr527	−1.05	0.00040
Slc6a3	−2.45	0.0010
RGD1561777	−0.82	0.0014
Mc3r	−1.03	0.0030
Tacr3/Nk3R	−0.83	0.0031
Chrna6	−1.76	0.0045
Slc47a2	−0.51	0.0047
Transcriptional regulation
Foxa1/HNF3-alpha	−0.78	0.00054	miR-106b, 194, 30b-5p/c, 20a
Mir181a-2	−0.69	0.00085
LOC690309	−0.78	0.0012	miR-26a, 29a/b/c, 222, 383
Pfdn1	−0.67	0.0014
Smg6	−0.51	0.0029
Other
Cry1	−0.58	0.0010
Samd9 l	−0.69	0.0038
Downregulated
Endosomes
Dnah3	0.63	0.00021
Lin7a/MALS-1	0.64	0.00043
LOC494539	0.51	0.0029	miR-125a-5p
Ifitm7	0.72	0.0039
Cell signaling
Dkk3	0.66	0.0011	let-7
Gtpbp4	0.78	0.0012
Membrane transporters/receptors
Olr625	0.64	0.00048
Vom1r2	0.69	0.0018
Vom1r26	0.93	0.0022
Olr1373	0.63	0.0033
Transcriptional regulation
Naca	0.57	0.00066
Other
LOC690000	0.53	0.00096
LOC691519	0.50	0.0018
LOC691988	0.84	0.0029
Senp17	1.34	0.0029
Apol3	1.14	0.0031
XTP2	0.90	0.0046

Canonical pathway^b	mRNA	p-value	RER
Ribosome biogenesis in eukaryotes	Rpp30, Gtpbp4	0.0024	27.45
Neuroactive ligand-receptor interaction	Tacr3, Chrna6, Mc3r	0.0019	12.27
RNA transport	Rpp30, Senp17	0.0081	14.78
Pathways in cancer	Hsp90ab1, Ret	0.0313	7.23

RER ratio of enrichment.
^aListed are miRNA transcripts significantly differentially expressed in the microarray experiment.
^bDetermined using WebGestalt.

ISSN: 1471-2202