Effect of blockade or stimulation of the EP1 receptor on ICH-induced brain injury. Antagonist (SC-51089, 10 µg/kg), agonist (17-pt-PGE2, 0.3 mg/kg), or vehicle (saline) was administered subcutaneously at the onset of injury, 6 h post-ICH, and at 12-h intervals thereafter. Seventy-two hours after ICH, brains were harvested and sections processed for Cresyl violet staining and lesion volume determination. a Representative photomicrographs of coronal brain sections from control (left panel), SC-51089- (middle panel), and 17-pt-PGE2- (right panel) treated mice. Scale bar 1 mm. b Quantification showed that SC-51089-treated mice had significantly more ICH-induced brain injury, whereas no significant differences were seen with 17-pt-PGE2 treatment. *p < 0.05 when compared to the control group, n = 8–10 per group.