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Figure 2 | BMC Neuroscience

Figure 2

From: NKCC1 downregulation induces hyperpolarizing shift of GABA responsiveness at near term fetal stages in rat cultured dorsal root ganglion neurons

Figure 2

Cl− load (a, b) and depletion (c, d) in DRGN at fetal stages E14 and E19. DRGN were held at −40 mV and 500 μM GABA was applied for 1 s every 30 s. Cl− load was induced by a 5 s GABA pulse (1.5 mM) during a depolarizing step to +70 mV while Cl− depletion was induced by applying the same pulse of GABA during a hyperpolarizing step to −100 mV. I GABA was normalized to the value obtained during the voltage step to +70 or −100 mV. The continuous lines show the fit of data during recovery using a single exponential function. Recovery time constants after Cl− load were 2.23 and 3.19 min at E14 (a) and E19 (b), respectively. Recovery time constants after Cl− depletion amounted to 0.79 and 1.5 min at E14 (c) and E19 (d), respectively. Insets I GABA recordings at times indicated by matched colors.

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BMC Neuroscience

ISSN: 1471-2202

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