Effect of GSNO on LKB1, ACC and AMPK phosphorylation status in ipsilateral (penumbra) area of IR brain. Western blot at 1 h of reperfusion showing phosphorylation of pLKB1 (a, a′), pACC and pAMPK/pACC (b, b′). GSNO was administered at 0 h after reperfusion began. Data are presented as mean ± SD (n = 5). ***p < 0.001, **p < 0.01 vs. Sham and GSNO.