Effect of GSNO on neuronal degeneration and axonal loss in ipsilateral (penumbra) area of IR brain at 4 h after reperfusion. Nissl staining a shows significant number of degenerating neurons (black arrow) in IR compared with GSNO and sham groups (intact neurons are shown by blue arrows). Bielshowsky silver and b H&E stainings c show loss of axonal and tissue integrity (red arrow), respectively, in IR compared with GSNO and sham. IR also shows pyknosis of neurons (black arrow). Photomicrographs are representative of n = 3 in each group.