Figure 5
GFP-Giα FRAP is unaffected by chronic antidepressant or mood stabilizer treatment. Giα-GFP was stably expressed in a C6 glioma cell line. Half-time to recovery of Giα-GFP is not altered by chronic treatment with escitalopram, lithium, or valproic acid. This suggests that the change in membrane compartmentalization of Gsα is not due to direct effects of these agents on lipid rafts, but that the effects of antidepressants are specific to Gsα. Sample size represents the number of cells assayed, with a minimum of 26 and a maximum of 46 cells assayed per experiment. Data were analyzed by one-way ANOVA followed by Tukey multiple comparison test for post hoc comparisons of means. Data are represented as mean ± SEM.