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Figure 4 | BMC Neuroscience

Figure 4

From: Differential effects of antidepressants escitalopram versus lithium on Gs alpha membrane relocalization

Figure 4

FRAP suggests that lithium combined with antidepressant does not reverse the effect of antidepressant GFP-Gsα was stably expressed in a C6 glioma cell line. Half-time to recovery of GFP-Gsα is increased after chronic escitalopram treatment, suggesting increased coupling with its effector, adenylyl cyclase. Lithium combined with escitalopram does not affect this value, suggesting an alternate locus of action for lithium. GFP-Gsα recovery is not altered by lithium or valproic acid treatment, suggesting unaltered coupling of GFP-Gsα with adenylyl cyclase. Sample size represents the number of cells assayed, with a minimum of 21 and a maximum of 101 cells assayed per experiment. Data were analyzed by one-way ANOVA (p < 0.0001) followed by Tukey multiple comparison test for post hoc comparisons of means. Data are represented as mean ± SEM (***, p < 0.0005 compared to vehicle).

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