Figure 2

Giα membrane compartmentalization is not altered by chronic antidepressant or lithium treatment. Purified lipid rafts isolated using sucrose gradient flotation revealed that neither chronic antidepressant treatment nor chronic lithium treatment move Giα out of lipid rafts. a The figure shows the percentage of change in Giα protein in the lipid raft membrane fractions compared to control (n = 4 for control, escitalopram, and lithium; n = 3 for valproate). Data were analyzed by one-way ANOVA followed by Tukey’s multiple comparison test for post hoc comparison of means. Data are represented as mean ± SEM b A representative immunoblot of lipid raft Gsα from the various treatment paradigms as explained in “Methods”. C control, E escitalopram, L lithium, V valproic acid.