Shox2 is required for the proper development of the facial motor nucleus. a Diagram of a sagittal section through the P0 brain highlights the regions represented in b–v. b X-gal stained sagittal sections through P0 brains show a decrease in staining levels in the facial motor nucleus (nVII) of Nestin-Cre; Shox2
lacZ/flox (B″) animals as compared to controls (B′). c–v ISH on P0 control (c, e, g, i, k, m, o, q, s, u) and Nestin-Cre; Shox2
flox/− (d, f, h, j, l, n, p, r, t, v) sagittal sections shows loss of Shox2 expression (compare c to d, dashed-circle), and decreases in Isl1 (compare e to f, dashed-circle), Phox2b (compare g to h, dashed-circle), Cntn2 (compare i to j, dashed-circle), Tubb3 (compare k to l, dashed-circle), Periph (compare m to n, dashed-circle), Slc18a3 (compare o to p, dashed-circle), Slit2 (compare q to r, dashed-circle), Shh (compare s to t, dashed-circle) and Ptch1 (compare u to v, dashed-circle) expression in the facial motor nucleus. nV trigeminal nucleus, RTN retrotrapezoid nucleus. Scale bar 250 μm.