Loss of Shox2 in the brain results in subtle changes in the expression pattern of Isl1 and Phox2b in the E14.5 facial motor nucleus. a Diagram of a sagittal section through the E14.5 brain highlights the regions represented in b–r. b X-gal stained sagittal sections through E14.5 embryos shows decreased staining in the facial motor nucleus (nVII) of Nestin-Cre; Shox2
lacZ/flox (B″) embryos that lack Shox2 function, as compared to controls (B′). c–r ISH on E14.5 control (c, e, g, i, k, m, o, q) and Nestin-Cre; Shox2
flox/− (d, f, h, j, l, n, p, r) sagittal sections shows loss of Shox2 expression (compare c to d, dashed-circle), and subtle changes in the spatial expression patterns of Isl1 (compare e to f, arrow), Phox2b (compare g to h, arrow), Cntn2 (compare i to j, dashed-circle), Tubb3 (compare k to l, dashed-circle), Periph (compare m to n, arrow), Slc18a3 (compare o to p, arrow) and Slit2 (compare q to r, arrow) in the developing facial motor nucleus. The dashed-circles in paired panels being compared (e.g. B′ and B″) are the same size, and this applies from B′ to r. Scale bar 250 μm.