Shox2 mRNA and lacZ expression in the developing embryonic face and postnatal facial motor nucleus. a, b Whole-mount in situ hybridization (WISH) at E11.5 (a) and E12.5 (b) show Shox2 expression in the trigeminal (V) and facial (VII, arrow) ganglia, in addition to the developing maxillary process (mp) and mandibular arch (ma). c–e In situ hybridization (ISH) on whole P0 brains (c, ventral viewpoint) and sagittal (d) or coronal (e) sections through the brain show Shox2 expression in the facial motor nucleus (nVII, arrows). f–j X-gal staining of Shox2
lacZ/+ embryos at E10.5 (f), E11.5 (g), E12.5 (h), E13.5 (i), and E14.5 (j) shows that Shox2 is expressed in the trigeminal and facial ganglion (f, g, arrow), in addition to the developing maxillary process and mandibular arch. Starting at E12.5 and continuing to E14.5, Shox2 expression becomes restricted to the trigeminal ganglion and the facial mesenchyme that will contribute to the maxilla and mandible, however it is absent from the facial ganglia. k–o X-gal staining of Shox2
lacZ/+ pups in the P0 face (k), brain (l, side viewpoint; m, ventral viewpoint) and sagittal (n) or coronal (o) sections through the brain show Shox2 expression in the tissue surrounding the facial nerves (k), with the exception of the nerves themselves, and Shox2 expression in the facial motor nucleus (l–o, arrows). ey eye, nV trigeminal nucleus, sbl superior buccolabial nerve, ibl inferior buccolabial nerve, mm marginal mandibular nerve, cb cerebellum, mb midbrain. Scale bar 500 μm.