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Table 2 Transport studies in HEK293 cells

From: Expression, sorting and transport studies for the orphan carrier SLC10A4 in neuronal and non-neuronal cell lines and in Xenopus laevis oocytes

Compound (5 µM)

NTCP

SLC10A4

NtNTCP-SLC10A4-CtNTCP

Uptake

Ratio

Uptake

Ratio

Uptake

Ratio

[3H]E-3-S

276.93 ± 29.12

10.1*

35.23 ± 2.01

1.3

27.91 ± 1.38

1.0

[3H]DHEAS

225.12 ± 16.51

6.9*

38.22 ± 0.90

1.2

31.84 ± 1.38

1.0

[3H]PREGS

1,656.09 ± 201.01

4.7*

366.06 ± 22.23

1.0

383.62 ± 17.38

1.0

 

DAT

SLC10A4

NtNTCP-SLC10A4-CtNTCP

[3H]Dopamine

1,578.17 ± 107.47

13.2*

128.61 ± 16.60

1.1

157.92 ± 19.11

1.3

[3H]Glutamate

  

22.60 ± 2.95

1.4

13.57 ± 1.62

0.8

[3H]Histamine

  

26.24 ± 1.40

1.3

25.68 ± 0.63

1.3

[3H]Acetyl-choline

  

352.00 ± 38.76

1.0

307.64 ± 31.41

0.9

[3H]Choline

  

929.53 ± 30.50

1.1

866.83 ± 23.63

1.0

  1. Values represent uptake in pmol/mg protein/30 min of the indicated compound as mean ± SD of quadruplicate determinations. Ratio represents uptake into carrier-expressing HEK293 cells divided by uptake into mock-transfected HEK293 cells. * Significantly different from control with p < 0.01.
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BMC Neuroscience

ISSN: 1471-2202

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