Expression, sorting and transport studies for the orphan carrier SLC10A4 in neuronal and non-neuronal cell lines and in Xenopus laevis oocytes

Table 1 Transport studies in Xenopus laevis oocytes

	SLC10A4	Control	Ratio
Uptake (pmol/oocyte/60 min)
[³H]Histamine (10 µM)	0.29 ± 0.02	0.27 ± 0.01	1.0
[³H]Aspartate (1 µM)	0.22 ± 0.01	0.28 ± 0.01	0.8
[³H]Acetate (25 µM)	8.79 ± 0.51	8.36 ± 0.72	1.0
[³H]Choline (1 µM)	2.68 ± 0.52	2.20 ± 0.19	1.2
[³H]GABA (1 µM)	0.52 ± 0.05	0.47 ± 0.04	1.1
[³H]Norepinephrine (1 µM)	0.16 ± 0.01	0.21 ± 0.02	0.8
[³H]Glutamate (1 µM)	0.014 ± 0.001	0.014 ± 0.002	1.0
Uptake (pmol/oocyte/30 min)
[³H]Acetylcholine (10 µM)	43.51 ± 3.62	37.51 ± 3.67	1.2
[³H]Dopamine (10 µM)	1.36 ± 0.06	1.43 ± 0.05	1.0
[³H]PREGS (1 µM)	1.19 ± 0.09	1.06 ± 0.09	1.1
[³H]DHEAS (1 µM)	0.043 ± 0.002	0.043 ± 0.004	1.0
[³H]E-3-S (1 µM)	0.050 ± 0.001	0.060 ± 0.005	0.8
Uptake (pmol/oocyte/10 min)
[³H]Lithocholic acid (0.3 µM)	3.68 ± 0.23	4.13 ± 0.07	0.9
[³H]Taurocholic acid (0.3 µM)	0.0158 ± 0.0009	0.0159 ± 0.0007	1.0
[³H]ATP (5 mM)	70.66 ± 9.06	68.84 ± 8.26	1.0
[³⁵S]Adenosine 5`-(γ-thio) triphosphate (5 mM)	69.76 ± 4.36	67.73 ± 2.53	1.0

Xenopus laevis oocytes were injected with human SLC10A4 cRNA or with water (control). Values represent uptake of the indicated compound as mean ± SD of at least 10 oocytes per group. Ratio represents uptake into SLC10A4 cRNA-injected oocytes divided by uptake into water-injected oocytes.

ISSN: 1471-2202