Skip to main content

Table 1 Summary of high throughput screening results

From: Targeted inhibition of the Shroom3–Rho kinase protein–protein interaction circumvents Nogo66 to promote axon outgrowth

Total # compounds screened

20,000

Hits from primary screen

180 (0.9%)

Dose response

36 (0.18%)

Available for re-supply

32 (0.16%)

Confirmed inhibitors

27 (0.14%)

IC50 <30 μM

9 (0.05%)

Enhanced neurite outgrowth

1 (0.005%)

  1. A 20,000 compound library was screened using the ELISA platform as described in Materials and Methods. 180 compounds were subject to dose response analysis. Of these 36 inhibited the Shroom3–ROCK interaction with pIC50 values greater than 4.0, had >60% efficacy at maximum dose tested, and had recovery rates in unrelated screens at <22%. 32 of the 36 chemicals were available for repurchase and of these 27 reconfirmed as inhibitors of the Shroom3–ROCK interaction. Nine compounds of the 27 confirmed hits have IC50 values less than 30 μM. One compound enhances neurite outgrowth.