Fig. 1

Cat Tas2r38 and Tas2r43 express on the cell surface similarly to their human orthologs. Each of the expression constructs was transfected individually along with Gα16-gust44. Expression of the receptors on the cell surface was determined 24 hours after transfection by flow cytometry of fixed cells, using a mAb recognizing the FLAG sequence encoded at the receptor N-terminus, which is exposed at the extracellular surface of TAS2R receptors. Shown are the fluorescence profiles for cells transfected with specific human (h) or cat (c) TAS2R expression constructs (black), or with vector alone (dotted line). Human TAS2R16 is included as a control as it consistently and robustly traffics to the cell surface.