UNC-2/CaV2 localization is independent of SLO-1/BK localization. slo-1 or ctn-1 null mutants do not have any obvious defect in UNC-2 localization. Left panels: representative image. Right panels: quantification of the puncta number. Data were analyzed by one-way ANOVA with Dunnett’s multiple comparisons test (wt vs. slo-1: P = 0.94; wt vs. ctn-1: P = 0.07).