Skip to main content
Figure 5 | BMC Neuroscience

Figure 5

From: Presynaptic BK channel localization is dependent on the hierarchical organization of alpha-catulin and dystrobrevin and fine-tuned by CaV2 calcium channels

Figure 5

The organization of SLO-1/BK at the presynaptic terminals is defective in unc-2 mutants. (A, B) An integrated SLO-1::GFP array cimIs10 was crossed with wild-type (A) or unc-2 (B) animals carrying the integrated RAB-3::mCherry array cimIs14. Arrowheads indicate SLO-1 puncta that are not co-localized with clusters of synaptic vesicles shown by RAB-3 puncta. The scale bars represent 5 μm. (C) Co-localization between SLO-1::GFP and RAB-3::mCherry in wild-type and unc-2 animals was analyzed with JACoP, an ImageJ plugin [44]. Manders’ coefficient M1 is defined as the ratio of the summed intensity of GFP pixels overlapped with mCherry to total GFP intensity, whereas M2 is conversely defined. M1 coefficients in wild-type (n = 5) and unc-2 (n = 4) animals are significantly different, whereas M2 coefficients in both animals are not different (Student t-test, n.s., p = 0.34; *p = 0.013). The data represent mean ± SEM.

Back to article page

BMC Neuroscience

ISSN: 1471-2202

Contact us