Figure 9From: Hydrogen improves neurological function through attenuation of blood–brain barrier disruption in spontaneously hypertensive stroke-prone ratsImmunofluorescence staining for matrix metalloproteinases (MMPs). Double staining for neuronal nuclear antigen (NeuN), glial fibrillary acidic protein (GFAP), and Iba-1 (green) showing that MMP-2 and MMP-9 (red) were expressed in the microglia (arrows), but not in neurons and astrocytes (nuclei, 4,6-diamidino-2-phenylindole [DAPI], blue). Co-localization is shown as yellow. Scale bars = 20 μm.Back to article page