Figure 7

Effect of hydrogen-rich water (HRW) on the permeability of the blood–brain barrier. a, Representative photomicrographs showing immunostaining for albumin. b, Quantification of the number of vessels with extravasated albumin in the cortex and hippocampus. In the cortex, there was no significant difference in numbers of vessels of extravasated albumin between the two groups. In the hippocampus, the HRW group had a significantly lower number of vessels with extravasated albumin (P < 0.001). Data were analyzed by an unpaired t-test. Values are expressed as mean ± SEM. n = 6 in each group. Scale bars = 100 μm. ***P < 0.001 vs. control group.