Figure 3

Effect of hydrogen-rich water (HRW) on cerebral lesions. Hematoxylin and eosin staining was performed in surviving animals at 16 weeks after treatment. Representative photographs showing bleeds in the cortex (a) and hippocampus (b), thromboses in the cortex (c) and hippocampus (d), and infarcts in the cortex (e) and hippocampus (f). The infarcted areas included cells with vacuole-like structures and/or liquefaction (asterisks). Quantification of cerebral bleeds and thromboses/infarcts in the cortex and hippocampus (g). The numbers of bleeds and thrombosis/infarcts were lower in the cortex and hippocampus in the HRW group. Data were analyzed by an unpaired t-test. Values are expressed as mean ± SEM. n = 3 in the control group, n = 6 in the HRW group. Scale bars = 50 μm (a, b, c, d) or 200 μm (e, f). *P < 0.05 vs. control group.