Figure 2

Current-blockade-dependent increase in the titer of Lv-Kir2.1. (A and B) The Lv-KirDN titer was higher than the Lv-Kir2.1 titer. The titers were determined with aliquots prepared before (unconcentrated) and after (concentrated) centrifugal concentration. The titers of unconcentrated and concentrated Lv-Kir-DN were both significantly higher than those of unconcentrated and concentrated Lv-Kir2.1 (n = 5; *p < 0.05, **p < 0.01, Student’s t test). (C and D) Blocking the Kir2.1 current increased the titer of Lv-Kir2.1. After plasmid transfection, the 293T cells were incubated in the presence or absence of 0.3 mM BaCl₂ for 48 h. The addition of BaCl₂ significantly increased the titers of both the unconcentrated and concentrated Lv-Kir2.1 (n = 5; p < 0.05, Student’s t test).