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Table 3 List of qPCR primers used to validate the sequencing data

From: Methamphetamine induces alterations in the long non-coding RNAs expression profile in the nucleus accumbens of the mouse

LncRNA   Primer sequence Tm(°C) Product length (bp)
AK036791 F 5′-TGGTGATTCTGTTACCGTCT-3′ 60 134
R 5′-AGGAGGTCCAAATACAAGAT-3′ 58
AK041753 F 5′-CTGTCCCTTGGTGATGCTGTT -3′ 62 132
R 5′-TGCACTGAAGCATTCTCTCTCC -3′ 62
AK046893 F 5′-CTCCTCCTCCTGGGAATGTCT -3′ 62 89
R 5′-CCCTAAATGTTCTCCTCCGTCTT -3′ 62
AK053003 F 5′-CTCAGGAGGTCATTCGG -3′ 50 199
R 5′-GAGAAACCTGGAGTAGTGG -3′ 49
AK034848 F 5′-TGATAAGGAGGGCGTCACAA -3′ 62 149
R 5′-ACCTTCACCAGCACCCAGA -3′ 62
AK019917 F 5′-CCCCTCTAAGCCTGGGAACT-3′ 62 133
R 5′-AACAAACCAACAAAAGCCACCT-3′ 62
AK085108 F 5′-AAACCAACAACAGGACCTCT-3′ 61 179
R 5′-ATTGGGAGTTTGATGCTTTC-3′ 62
AK048817 F 5′-AGGATGTGAGTGGACTGTGG-3′ 62 148
R 5′-TTTGGTTGTCAGAGATGGCT-3′ 62
AK081238 F 5′-TGTTGTGGGTATATGTGGAT-3′ 58 137
R 5′-ACAGTGAAATAAGATGGACC-3′ 56
AK080587 F 5′-AGCAGAGGATGTATCAAAGC-3′ 59 109
R 5′-ACCAAAGGGACTGACAGAAT-3′ 60
AK003846 F 5′-GTGTGCTCCAGAAAGTGTAA-3′ 58 131
R 5′-GAGAAGAAGAGAGAGGTTGC-3′ 58
AK142426 F 5′-TTTTGGGAGGGTGAGGG -3′ 56 177
R 5′-GAACGGTGAAGGCGACA-3′ 55
GAPDH F 5′-TGTGTCCGTCGTGGATCTGA-3′ 55 150
R 5′-TTGCTGTTGAAGTCGCAGGAG-3′ 52
  1. The primer pairs were chosen to maintain the melt temperature (Tm) between 45°C and 62°C and the product lengths between 80 and 200 bp.