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Table 3 List of qPCR primers used to validate the sequencing data

From: Methamphetamine induces alterations in the long non-coding RNAs expression profile in the nucleus accumbens of the mouse

LncRNA

 

Primer sequence

Tm(°C)

Product length (bp)

AK036791

F

5′-TGGTGATTCTGTTACCGTCT-3′

60

134

R

5′-AGGAGGTCCAAATACAAGAT-3′

58

AK041753

F

5′-CTGTCCCTTGGTGATGCTGTT -3′

62

132

R

5′-TGCACTGAAGCATTCTCTCTCC -3′

62

AK046893

F

5′-CTCCTCCTCCTGGGAATGTCT -3′

62

89

R

5′-CCCTAAATGTTCTCCTCCGTCTT -3′

62

AK053003

F

5′-CTCAGGAGGTCATTCGG -3′

50

199

R

5′-GAGAAACCTGGAGTAGTGG -3′

49

AK034848

F

5′-TGATAAGGAGGGCGTCACAA -3′

62

149

R

5′-ACCTTCACCAGCACCCAGA -3′

62

AK019917

F

5′-CCCCTCTAAGCCTGGGAACT-3′

62

133

R

5′-AACAAACCAACAAAAGCCACCT-3′

62

AK085108

F

5′-AAACCAACAACAGGACCTCT-3′

61

179

R

5′-ATTGGGAGTTTGATGCTTTC-3′

62

AK048817

F

5′-AGGATGTGAGTGGACTGTGG-3′

62

148

R

5′-TTTGGTTGTCAGAGATGGCT-3′

62

AK081238

F

5′-TGTTGTGGGTATATGTGGAT-3′

58

137

R

5′-ACAGTGAAATAAGATGGACC-3′

56

AK080587

F

5′-AGCAGAGGATGTATCAAAGC-3′

59

109

R

5′-ACCAAAGGGACTGACAGAAT-3′

60

AK003846

F

5′-GTGTGCTCCAGAAAGTGTAA-3′

58

131

R

5′-GAGAAGAAGAGAGAGGTTGC-3′

58

AK142426

F

5′-TTTTGGGAGGGTGAGGG -3′

56

177

R

5′-GAACGGTGAAGGCGACA-3′

55

GAPDH

F

5′-TGTGTCCGTCGTGGATCTGA-3′

55

150

R

5′-TTGCTGTTGAAGTCGCAGGAG-3′

52

  1. The primer pairs were chosen to maintain the melt temperature (Tm) between 45°C and 62°C and the product lengths between 80 and 200 bp.