Figure 6

Pod1 increases survival to starvation in pod1 KD flies. (A) Relative transcript levels of pod1 were measured using real-time PCR in male flies kept at 29°C to verify the efficiency reduced levels of pod1 mRNA in homozygous pod1 ^−/− and heterozygous pod1 ^+/− flies. Flies were aged 5–7 days at 29°C after eclosion. Real-time PCR was repeated 5 times. Statistical significance was validated using one-way ANOVA where *P < 0.05. (B,C) We tested the effect of pod1-knockdown in flies on survival assay. Pod1 ^+/–and pod1 ^−/− were placed in a vial containing 1% agarose and maintained at both 24°C (B) and 29°C (C). Percent survival was calculated every 12 h (n=75-95 flies per genotype); *P < 0.05 validated through one-way ANOVA with appropriate post-hoc test for multiple comparisons. (D) To validate how nutritional state affects the expression levels of pod1 mRNA in flies they RNA was extracted from flies during different nutritional states. Error bars indicate SEM. Significance levels are indicated: *P < 0.05; **P < 0.01; ***P <0.001.