Figure 2

Cytoprotective effect of GH. The protective effect of GH with GSNO pretreatment was observed in mutant cells (A). In homocysteine-treated cells, reduced viabilities in A4V were partly reversed by GH at 0.4 I.U/ml (p = 0.046) (B). GSNO treatment increased nuclear fragmentation; GH treatment reduced this phenomenon. Homocysteine also reduced cell viability and the number of fragmented nuclei; this was reversed by pretreatment with GH (p = 0.039) (C). GSNO treatment increased 116 kD and 24 kD PARP fragments in A4V and G93A cells, which were reduced by GH treatment (D). Results are expressed as mean values and SD (*p < 0.05).