Table 4 Gradient conditions for LC
From: Quantitative detection of free 24S-hydroxycholesterol, and 27-hydroxycholesterol from human serum
Step | Total time (min) | Flow rate (ÎĽl/min) | Mobile phase A* (%) | Mobile phase B* (%) |
|---|---|---|---|---|
0 | 0 | 300 | 100 | 0 |
1 | 0.3 | 300 | 15 | 85 |
2 | 9 | 300 | 0 | 100 |
3 | 12 | 300 | 100 | 0 |