Table 2 Precision and accuracy of hydroxycholesterols
From: Quantitative detection of free 24S-hydroxycholesterol, and 27-hydroxycholesterol from human serum
Concentration (ng/ml) | n | Variables | hydroxycholesterols | |
|---|---|---|---|---|
| Â | Â | Â | 24S-hydroxycholesterol | 27-hydroxycholesterol |
| Â | Â | Â | a. Inter-day analysis | |
50 | 5 | Mean (ng/ml) | 50.7 | 56.9 |
| Â | Â | SD (ng/ml) | 4.5 | 2.2 |
| Â | Â | CV (%) | 8.9 | 3.9 |
| Â | Â | Accuracy (%) | 101.4 | 113.6 |
100 | 5 | Mean (ng/ml) | 91.0 | 97.5 |
| Â | Â | SD (ÎĽg/ml) | 4.7 | 8.5 |
| Â | Â | CV (%) | 5.1 | 8.7 |
| Â | Â | Accuracy (%) | 91.0 | 97.5 |
| Â | Â | Â | b. Intra-day analysis | |
50 | 5 | Mean (ng/ml) | 49.4 | 59.0 |
| Â | Â | SD (ng/ml) | 5.8 | 0.9 |
| Â | Â | CV (%) | 11.8 | 1.6 |
| Â | Â | Accuracy (%) | 98.8 | 118.2 |
100 | 5 | Mean (ÎĽg/ml) | 98.0 | 94.9 |
| Â | Â | SD (ng/ml) | 3.3 | 4.1 |
| Â | Â | CV (%) | 3.4 | 4.3 |
| Â | Â | Accuracy (%) | 98.0 | 94.9 |