Quantitative detection of free 24S-hydroxycholesterol, and 27-hydroxycholesterol from human serum

BMC Neuroscience

Table 1 Molecular and fragment ion m/z, and associated parameters for detection and quantification of 24S-hydroxycholesterol, 27-hydroxycholesterol and 24(R/S) hydroxycholesterol (d6) individual species by MRM

Anylate	Mol. Wt.	Molecular ion	Fragment ion	Time	DP*	FP*	Dwell*	CE*	CXP*
24S-hydroxycholesterol	402.66	420.3	385.5	10.48	60	400	150	13	5
27-hydroxycholesterol	402.66	420.3	385.5	10.83	60	400	150	13	5
24(R/S) hydroxycholesterol (d6)	408.69	426.4	373.6	10.47	45	250	150	20	10

*DP = Declustereing potential (eV), FP = focusing potential (eV), Dwell = Dwell time (ms), CE = Collision energy (eV), CXP = Collision exit potential (eV).

ISSN: 1471-2202