Figure 3
From: Quantitative detection of free 24S-hydroxycholesterol, and 27-hydroxycholesterol from human serum
Chromatograms for 24S-hydroxycholesterol and 27-hydroxycholesterol. LC/MS/MS chromatograms for A) 24S-hydroxycholesterol and 27-hydroxycholesterol and B) 24(R/S)-hydroxycholesterol (d6). Base line chromatogram for C) methanol. D) Chromatogram for 24S-hydroxycholesterol (10.45) and 27-hydroxycholesterol (10.83) extracted from serum. E) Chromatogram for 24S-hydroxycholesterol and 27-hydroxycholesterols spiked serum sample. F) Chromatogram of 24(R/S)-hydroxycholesterol (d6) added into and extracted from serum sample.