Effect of NGEN on whole-cell I
in α -hSlo -expressing HEK293T cells. In these experiments, cells were bathed in normal Tyrode’s solution containing 1.8 mM CaCl2. (A) Current traces in response to membrane depolarization from 0 to +50 mV. The upper part indicates the voltage protocol used. a: control; b: 3 μM NGEN; c: 10 μM NGEN; and d: 30 μM NGEN. (B) Summary of the data showing effects of NGEN, NGEN plus verruculogen (Verr; 1 μM) and NGEN plus iberiotoxin (Iber; 200 nM) on I K(Ca) amplitude in these cells (mean ± SEM; n = 10-13 for each bar). *Significantly different from control. **Significantly different from NGEN (30 μM) alone group.